A molecular beacon-based real-time NASBA assay for detection ofMycobacterium aviumsubsp.paratuberculosisin water and milk
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چکیده
منابع مشابه
molecular beacon probes-base multiplex nasba real-time for detection of hiv-1 and hcv.
developed in 1991, nucleic acid sequence-based amplification (nasba) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than pcr, and it can also be more sensitive. this paper describes the development of a molecular beacon-based multiplex nasba assay for simultaneous detection of hiv-1 and hcv in plasma samples.a well-conserved region...
متن کاملA molecular beacon-based real time NASBA assay for detection of Listeria monocytogenes in food products: role of target mRNA secondary structure on NASBA design.
A molecular beacon-based real-time NASBA (QNASBA) assay for detection and identification of Listeria monocytogenes has been developed. A correlation between targeting highly accessible mRNA sequences and QNASBA efficiency and sensitivity was demonstrated. The assay targets a sequence from the mRNA transcript of the hly gene which is specific for this bacterium; and includes an internal amplific...
متن کاملMolecular beacon probes–base multiplex NASBA Real-time for detection of HIV-1 and HCV
BACKGROUND AND OBJECTIVES Developed in 1991, nucleic acid sequence-based amplification (NASBA) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than PCR, and it can also be more sensitive. This paper describes the development of a molecular beacon-based multiplex NASBA assay for simultaneous detection of HIV-1 and HCV in plasma sampl...
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Nucleic acid sequence-based amplification (NASBA) was applied in combination with a fluorescein-conjugated molecular beacon specific for a sequence flanked by transcript-specific primers in order to monitor hblC enterotoxin gene expression in real-time from milk separately contaminated with Bacillus amyloliquefaciens, Bacillus cereus, and Bacillus circulans. Maximal enterotoxin expression was n...
متن کاملMolecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA.
Molecular beacon probes can be employed in a NASBA amplicon detection system to generate a specific fluorescent signal concomitantly with amplification. A molecular beacon, designed to hybridize within the target sequence, was introduced into NASBA reactions that amplify the genomic RNA of potato leafroll virus (PLRV). During amplification, the probe anneals to the antisense RNA amplicon genera...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2004
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2004.tb09686.x